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KMID : 1094720170220020129
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Biotechnology and Bioprocess Engineering
2017 Volume.22 No. 2 p.129 ~ p.135
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Functional characterization of thioredoxin h type 5 with antimicrobial activity from Arabidopsis thaliana
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Park Seong-Cheol
Jung Young-Jun
Kim Il-Ryong
Lee Yong-Jae
Kim Young-Min
Lee Jung-Ro
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Abstract
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A plant protein with effective antifungal activity was isolated from Arabidopsis thaliana leaves. The results of N-terminal sequencing analysis revealed that the isolated protein matches with a partial fragment of A. thaliana oxidoreductase thioredoxin h type 5; AtTrx-h5 (GenBank accession number AT1G45145). The AtTrx-h5 gene isolated from an Arabidopsis leaf cDNA library was transformed into Escherichia coli to investigate the functional characterizations of AtTrx-h5 protein. Recombinant AtTrx-h5 protein inhibited the conidia germination in 7 filamentous fungal cells and the proliferation in 3 pathogenic yeast cells. In addition, the cellular distribution of recombinant AtTrx-h5 protein in Fusarium solani showed pH-dependent cytosolic accumulation. Interestingly, AtTrx-h5 acted as an inhibitor of fungal growth via cellular reactive oxygen species (ROS) generation. We propose the novel functions of AtTrx-h5 as a potential candidate for natural antifungal material in the study.
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KEYWORD
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thioredoxin, AtTrx-h5, reductase, antifungal activity
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